Discovery of Novel High-Molecular Weight Oligosaccharides Containing N-Acetylhexosamine in Bovine Colostrum Whey Permeate Hydrolyzed with Aspergillus oryzae β-Galactosidase
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چکیده
منابع مشابه
Synthesis of Oligosaccharides Derived from Lactulose (OsLu) Using Soluble and Immobilized Aspergillus oryzae β-Galactosidase
β-Galactosidase from Aspergillus oryzae offers a high yield for the synthesis of oligosaccharides derived from lactulose (OsLu) by transgalactosylation. Oligosaccharides with degree of polymerization (DP) ≥ 3 have shown to possess higher in vitro bifidogenic effect than di- and tetrasaccharides. Thus, in this work, an optimization of reaction conditions affecting the specific selectivity of A. ...
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The synthesis of galacto-oligosaccharides (GOS) by β-galactosidase immobilized in both polyvinyl alcohol (PVA) lenses and sol-gel carriers was studied and compared with the performance of the free enzyme. PVA-immobilized β-galactosidase retained 95 % of the initial activity after seven repeated uses and retained 51 % of the initial activity after 3 months of storage, while sol-gel-immobilized β...
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To test the hypothesis that a low molecular weight fraction of colostral whey could affect the morphology and barrier function of the small intestine, 30 3-d-old piglets (normal or low birth weight) were suckled (n = 5), artificially fed with milk formula (n = 5), or artificially fed with milk formula with a low molecular weight fraction of colostral whey (n = 5) until 10 d of age. The small in...
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Low energy ultrasound irradiation (20 kHz, 1.0 W·L) was applied to enhance bioethanol production from whey ultrafiltration permeate by co-immobilized Saccharomyces cerevisiae cells and β-galactosidese enzyme. Sugar utilization and ethanol formation were investigated as a function of hydraulic retention time (HRT) between 12 and 36 h. Maximum ethanol production under HRT of 36 h was 26.30 g·L wi...
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Different compositions of liquid media were used for their efficacy in the production of beta-galactosidase by Aspergillus oryzae in submerged fermentation. Enzyme production was improved by switching from lactose-based media to soybean meal- and wheat bran-based media. Further improvement was made when the soybean meal medium was supplemented with sodium nitrate and magnesium sulfate. Res...
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ژورنال
عنوان ژورنال: Journal of Agricultural and Food Chemistry
سال: 2019
ISSN: 0021-8561,1520-5118
DOI: 10.1021/acs.jafc.8b06965